PFD6 (PFDN6) Mouse Monoclonal Antibody [Clone ID: OTI4E10]

CAT#: CF504110

Carrier-free (BSA/glycerol-free) PFDN6 mouse monoclonal antibody, clone OTI4E10 (formerly 4E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFDN6 (RC207901, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFDN6. Positive lysates LY415391 (100ug) and LC415391 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PFDN6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504110)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PFDN6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504110)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PFDN6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504110)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PFDN6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504110)

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  HEK293T cells transfected with either RC207901 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PFDN6 antibody (TA504110), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4E10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-129 of human PFDN6(NP_055075) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.4 kDa
• Gene Name: Homo sapiens prefoldin subunit 6 (PFDN6), transcript variant 2, mRNA.
• Database Link:
  NP_055075
  Entrez Gene 14976 MouseEntrez Gene 309629 RatEntrez Gene 10471 Human
  UniProt ID O15212
• Background: PFDN6 is a subunit of the heteromeric prefoldin complex that chaperones nascent actin (see MIM 102560) and alpha- and beta-tubulin (see MIM 602529 and MIM 191130, respectively) chains pending their transfer to the cytosolic chaperonin containing TCP1 (MIM 186980) (CCT) complex (Hansen et al., 1999 [PubMed 10209023]). [supplied by OMIM]
• Synonyms: H2-KE2; HKE2; KE-2; PFD6

Reference Data

• Protein Families: Stem cell - Pluripotency