GIRK1 (KCNJ3) Mouse Monoclonal Antibody [Clone ID: OTI2F2]

CAT#: CF504137

Carrier-free (BSA/glycerol-free) KCNJ3 mouse monoclonal antibody, clone OTI2F2 (formerly 2F2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCNJ3 (RC205322, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCNJ3. Positive lysates LY400811 (100ug) and LC400811 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC205322 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KCNJ3 antibody (TA504137), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2F2
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 279-501 of human KCNJ3(NP_002230) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.4 kDa
• Gene Name: Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 1, mRNA.
• Database Link:
  NP_002230
  Entrez Gene 16519 MouseEntrez Gene 50599 RatEntrez Gene 3760 Human
  UniProt ID P48549
• Background: Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. [provided by RefSeq]
• Synonyms: GIRK1; KGA; KIR3.1

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane