Hsp75 (TRAP1) Mouse Monoclonal Antibody [Clone ID: OTI1A1]

CAT#: CF504224

Carrier-free (BSA/glycerol-free) TRAP1 mouse monoclonal antibody, clone OTI1A1 (formerly 1A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP1 (RC203439, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP1. Positive lysates LY402533 (100ug) and LC402533 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP1 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-TRAP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TRAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504224)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504224)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504224)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504224)

• Anti-TRAP1 mouse monoclonal antibody (TA504224) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP1 (RC203439).

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Specifications

Product Data

• Clone Name: OTI1A1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full lengt human recombinant protein of human TRAP1(NP_057376) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79.9 kDa
• Gene Name: Homo sapiens TNF receptor associated protein 1 (TRAP1), transcript variant 1, mRNA.
• Database Link:
  NP_057376
  Entrez Gene 68015 MouseEntrez Gene 287069 RatEntrez Gene 102153194 DogEntrez Gene 706234 MonkeyEntrez Gene 10131 Human
  UniProt ID Q12931
• Background: HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]). [supplied by OMIM]
• Synonyms: HSP 75; HSP75; HSP90L; TRAP-1

Reference Data

• Protein Families: Druggable Genome