ALG2 Mouse Monoclonal Antibody [Clone ID: OTI3C2]

CAT#: CF504242

Carrier-free (BSA/glycerol-free) ALG2 mouse monoclonal antibody, clone OTI3C2 (formerly 3C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2. Positive lysates LY409737 (100ug) and LC409737 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALG2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504242)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504242)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504242)

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Specifications

Product Data

• Clone Name: OTI3C2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALG2(NP_149078) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.9 kDa
• Gene Name: Homo sapiens ALG2 alpha-1,3/1,6-mannosyltransferase (ALG2), transcript variant 1, mRNA.
• Database Link:
  NP_149078
  Entrez Gene 85365 Human
  UniProt ID Q9H553
• Background: This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: CDGIi; hALPG2; NET38

Reference Data

• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis