PECI (ECI2) Mouse Monoclonal Antibody [Clone ID: OTI1C8]

CAT#: CF504275

Carrier-free (BSA/glycerol-free) PECI mouse monoclonal antibody, clone OTI1C8 (formerly 1C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECI (RC204994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECI. Positive lysates LY401844 (100ug) and LC401844 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PECI monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504275)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504275)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504275)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PECI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504275)

• Anti-PECI mouse monoclonal antibody (TA504275) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECI (RC204994).

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Specifications

Product Data

• Clone Name: OTI1C8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PECI(NP_006108) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40 kDa
• Gene Name: Homo sapiens enoyl-CoA delta isomerase 2 (ECI2), transcript variant 1, mRNA.
• Database Link:
  NP_006108
  Entrez Gene 23986 MouseEntrez Gene 291075 RatEntrez Gene 478706 DogEntrez Gene 709579 MonkeyEntrez Gene 10455 Human
  UniProt ID O75521
• Background: PECI is an auxiliary enzyme that catalyzes an isomerization step required for the beta-oxidation of unsaturated fatty acids. [supplied by OMIM]
• Synonyms: ACBD2; dJ1013A10.3; DRS-1; DRS1; HCA88; PECI

Reference Data

• Protein Pathways: Fatty acid metabolism