RUVBL2 Mouse Monoclonal Antibody [Clone ID: OTI1A6]

CAT#: CF504281

Carrier-free (BSA/glycerol-free) RUVBL2 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (RC200933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2. Positive lysates LY401993 (100ug) and LC401993 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RUVBL2 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-RUVBL2 monoclonal antibody (1:200).

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-RUVBL2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504281)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504281)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504281)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504281)

• Anti-RUVBL2 mouse monoclonal antibody (TA504281) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RUVBL2 (RC200933).

• HEK293T cells transfected with either RC200933 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RUVBL2 antibody (TA504281), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragmengt corresponding to amino acids 113-370 of human RUVBL2(NP_006657) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51 kDa
• Gene Name: Homo sapiens RuvB like AAA ATPase 2 (RUVBL2), transcript variant 1, mRNA.
• Database Link:
  NP_006657
  Entrez Gene 20174 MouseEntrez Gene 292907 RatEntrez Gene 476418 DogEntrez Gene 100428794 MonkeyEntrez Gene 10856 Human
  UniProt ID Q9Y230
• Background: This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]
• Synonyms: CGI-46; ECP51; INO80J; REPTIN; RVB2; TIH2; TIP48; TIP49B

Reference Data

• Protein Families: Transcription Factors