PGP9.5 (UCHL1) Mouse Monoclonal Antibody [Clone ID: OTI2B5]

CAT#: CF504289

Carrier-free (BSA/glycerol-free) UCHL1 mouse monoclonal antibody, clone OTI2B5 (formerly 2B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UCHL1 (RC201803, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UCHL1. Positive lysates LY401345 (100ug) and LC401345 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UCHL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504289)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504289)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504289)

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  HEK293T cells transfected with either RC201803 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UCHL1 antibody (TA504289), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2B5
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:50, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UCHL1(NP_004172) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.6 kDa
• Gene Name: Homo sapiens ubiquitin C-terminal hydrolase L1 (UCHL1), mRNA.
• Database Link:
  NP_004172
  Entrez Gene 22223 MouseEntrez Gene 29545 RatEntrez Gene 701579 MonkeyEntrez Gene 7345 Human
  UniProt ID P09936
• Background: The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease. [provided by RefSeq, Sep 2009]
• Synonyms: HEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Parkinson's disease