Frataxin (FXN) Mouse Monoclonal Antibody [Clone ID: OTI3C7]

CAT#: CF504335

Carrier-free (BSA/glycerol-free) FXN mouse monoclonal antibody, clone OTI3C7 (formerly 3C7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FXN (RC204880, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FXN. Positive lysates LY400054 (100ug) and LC400054 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3C7
• Applications: WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FXN(NP_000135) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.8 kDa
• Gene Name: Homo sapiens frataxin (FXN), transcript variant 1, mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_000135
  Entrez Gene 2395 Human
  UniProt ID Q16595
• Background: This nuclear gene encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: CyaY; FA; FARR; FRDA; X25

Reference Data

• Protein Families: Druggable Genome