Calpain 2 (CAPN2) Mouse Monoclonal Antibody [Clone ID: OTI1F4]

CAT#: CF504345

Carrier-free (BSA/glycerol-free) CAPN2 mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)

Product Images

HEK293T cells transfected with either RC205642 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CAPN2 antibody (TA504345), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-CAPN2 antibody (TA504345), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-CAPN2 antibody (TA504345), (Red), compared to a nonspecific negative control antibody, (Blue).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN2 (RC205642, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN2. Positive lysates LY400662 (100ug) and LC400662 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and CAPN2-Knockout 293T cells (KO, Cat# LC841490) were separated by SDS-PAGE and immunoblotted with anti-CAPN2 monoclonal antibody TA504345, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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Specifications

Product Data

• Clone Name: OTI1F4
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79.8 kDa
• Gene Name: Homo sapiens calpain 2 (CAPN2), transcript variant 1, mRNA.
• Database Link:
  NP_001739
  Entrez Gene 12334 MouseEntrez Gene 29154 RatEntrez Gene 703047 MonkeyEntrez Gene 824 Human
  UniProt ID P17655
• Background: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
• Synonyms: CANP2; CANPL2; CANPml; mCANP

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis, Focal adhesion