AFG3L2 Mouse Monoclonal Antibody [Clone ID: OTI2D3]

CAT#: CF504404

Carrier-free (BSA/glycerol-free) AFG3L2 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFG3L2 (RC209058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFG3L2. Positive lysates LY416420 (100ug) and LC416420 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2D3
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-250 of human AFG3L2(NP_006787) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88.3 kDa
• Gene Name: Homo sapiens AFG3 like matrix AAA peptidase subunit 2 (AFG3L2), mRNA.
• Database Link:
  NP_006787
  Entrez Gene 69597 MouseEntrez Gene 307350 RatEntrez Gene 10939 Human
  UniProt ID Q9Y4W6
• Background: This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders. [provided by RefSeq, Jul 2008]
• Synonyms: SCA28; SPAX5

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane