PDRG1 Mouse Monoclonal Antibody [Clone ID: OTI4F12]

CAT#: CF504411

Carrier-free (BSA/glycerol-free) PDRG1 mouse monoclonal antibody, clone OTI4F12 (formerly 4F12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDRG1 (RC203661, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDRG1. Positive lysates LY410685 (100ug) and LC410685 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDRG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504411)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDRG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504411)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDRG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504411)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDRG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504411)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDRG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504411)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDRG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504411)

• Flow cytometric Analysis of Hela cells, using anti-PDRG1 antibody (TA504411), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-PDRG1 antibody (TA504411), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4F12
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDRG1(NP_110442) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15.3 kDa
• Gene Name: Homo sapiens p53 and DNA damage regulated 1 (PDRG1), mRNA.
• Database Link:
  NP_110442
  Entrez Gene 68559 MouseEntrez Gene 296278 RatEntrez Gene 81572 Human
  UniProt ID Q9NUG6
• Synonyms: C20orf126; PDRG