IGFBP2 Mouse Monoclonal Antibody [Clone ID: OTI1A7]

CAT#: CF504413

Carrier-free (BSA/glycerol-free) IGFBP2 mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP2 (RC202573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP2. Positive lysates LY424614 (100ug) and LC424614 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-IGFBP2 monoclonal antibody (1:200).

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  HEK293T cells transfected with either RC202573 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGFBP2 antibody (TA504413), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1A7
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IGFBP2(NP_000588) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens insulin like growth factor binding protein 2 (IGFBP2), transcript variant 1, mRNA.
• Database Link:
  NP_000588
  Entrez Gene 16008 MouseEntrez Gene 25662 RatEntrez Gene 3485 Human
  UniProt ID P18065
• Synonyms: IBP2; IGF-BP53

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein