S100P Mouse Monoclonal Antibody [Clone ID: OTI1A4]

CAT#: CF504478

Carrier-free (BSA/glycerol-free) S100P mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100P (RC201533, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100P. Positive lysates LY401809 (100ug) and LC401809 (20ug) can be purchased separately from OriGene.

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  Anti-S100P mouse monoclonal antibody (TA504478) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY S100P (RC201533).

Specifications

Product Data

• Clone Name: OTI1A4
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human S100P(NP_005971) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 10.2 kDa
• Gene Name: Homo sapiens S100 calcium binding protein P (S100P), mRNA.
• Database Link:
  NP_005971
  Entrez Gene 6286 Human
  UniProt ID P25815
• Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. [provided by RefSeq]
• Synonyms: MIG9