TMEFF2 Mouse Monoclonal Antibody [Clone ID: OTI3H8]

CAT#: CF504482

Carrier-free (BSA/glycerol-free) TMEFF2 mouse monoclonal antibody, clone OTI3H8 (formerly 3H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEFF2 (RC203736, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEFF2. Positive lysates LY402517 (100ug) and LC402517 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC203736 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TMEFF2 antibody (TA504482), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3H8
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TMEFF2(NP_057276) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.2 kDa
• Gene Name: Homo sapiens transmembrane protein with EGF like and two follistatin like domains 2 (TMEFF2), transcript variant 1, mRNA.
• Database Link:
  NP_057276
  Entrez Gene 23671 Human
  UniProt ID Q9UIK5
• Synonyms: CT120.2; HPP1; TENB2; TPEF; TR; TR-2

Reference Data

• Protein Families: Transmembrane