BACH1 Mouse Monoclonal Antibody [Clone ID: OTI4E11]

CAT#: CF504484

Carrier-free (BSA/glycerol-free) BACH1 mouse monoclonal antibody, clone OTI4E11 (formerly 4E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BACH1 (RC221628, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BACH1. Positive lysates LY404144 (100ug) and LC404144 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and BACH1-Knockout HeLa cells (KO, Cat# LC835403) were separated by SDS-PAGE and immunoblotted with anti-BACH1 monoclonal antibody TA504484 (1:2000). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BACH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504484)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BACH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504484)

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  HEK293T cells transfected with either RC221628 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BACH1 antibody (TA504484), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4E11
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BACH1(NP_996749) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.8 kDa
• Gene Name: Homo sapiens BTB domain and CNC homolog 1 (BACH1), transcript variant 1, mRNA.
• Database Link:
  NP_996749
  Entrez Gene 571 Human
  UniProt ID O14867
• Background: This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq]
• Synonyms: BACH-1; BTBD24

Reference Data

• Protein Families: Transcription Factors