PSMA (FOLH1) Mouse Monoclonal Antibody [Clone ID: OTI2E1]

CAT#: CF504509

Carrier-free (BSA/glycerol-free) FOLH1 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOLH1 (RC218310, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOLH1. Positive lysates LY429203 (100ug) and LC429203 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FOLH1 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-FOLH1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

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  Standard curve for ELISA analysis with FOLH1 recombinant protein (dilution range from 5ng/ml to 5ug/ml) using FOLH1 Capture Antibody (Cat# TA504509) at 5ug/ml and HRP conjugated FOLH1 Detection mAb (Cat# TA504550) at 0.03ug/ml.

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  Anti-FOLH1 mouse monoclonal antibody (TA504509) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOLH1 (RC218310).

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Specifications

Product Data

• Clone Name: OTI2E1
• Applications: ELISA, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FOLH1(NP_004467) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.2 kDa
• Gene Name: Homo sapiens folate hydrolase 1 (FOLH1), transcript variant 1, mRNA.
• Database Link:
  NP_004467
  Entrez Gene 53320 MouseEntrez Gene 85309 RatEntrez Gene 476775 DogEntrez Gene 707714 MonkeyEntrez Gene 2346 Human
  UniProt ID Q04609
• Background: This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq]
• Synonyms: FGCP; FOLH; GCP2; GCPII; mGCP; NAALAD1; NAALAdase; PSM; PSMA

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane