LRRC50 (DNAAF1) Mouse Monoclonal Antibody [Clone ID: OTI4H3]

CAT#: CF504578

Carrier-free (BSA/glycerol-free) LRRC50 mouse monoclonal antibody, clone OTI4H3 (formerly 4H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50. Positive lysates LY405926 (100ug) and LC405926 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Clone Name: OTI4H3
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LRRC50(NP_848547) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79.8 kDa
• Gene Name: Homo sapiens dynein axonemal assembly factor 1 (DNAAF1), transcript variant 1, mRNA.
• Database Link:
  NP_848547
  Entrez Gene 68270 MouseEntrez Gene 361419 RatEntrez Gene 715137 MonkeyEntrez Gene 123872 Human
  UniProt ID Q8NEP3
• Background: The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. COMPLETENESS: complete on the 3' end.
• Synonyms: CILD13; LRRC50; ODA7; swt