SERPINE2 Mouse Monoclonal Antibody [Clone ID: OTI2C9]

CAT#: CF504611

Carrier-free (BSA/glycerol-free) SERPINE2 mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SERPINE2 (Cat# RC204904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2(Cat# TA504611). Positive lysates LY416795 (100ug) and LC416795 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINE2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-SERPINE2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINE2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINE2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINE2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• HEK293T cells transfected with either RC204904 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINE2 antibody (TA504611), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2C9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SERPINE2(NP_006207) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.8 kDa
• Gene Name: serpin family E member 2
• Database Link:
  NP_006207
  Entrez Gene 20720 MouseEntrez Gene 5270 Human
  UniProt ID P07093
• Synonyms: GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI

Reference Data

• Protein Families: Druggable Genome, Secreted Protein