SRPR beta (SRPRB) Mouse Monoclonal Antibody [Clone ID: OTI2D4]

CAT#: CF504724

Carrier-free (BSA/glycerol-free) SRPRB mouse monoclonal antibody, clone OTI2D4 (formerly 2D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRPRB (RC209486, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRPRB. Positive lysates LY402853 (100ug) and LC402853 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SRPRB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SRPRB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504724)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SRPRB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504724)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SRPRB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504724)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SRPRB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504724)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SRPRB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504724)

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Specifications

Product Data

• Clone Name: OTI2D4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SRPRB(NP_067026) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.5 kDa
• Gene Name: Homo sapiens SRP receptor subunit beta (SRPRB), transcript variant 1, mRNA.
• Database Link:
  NP_067026
  Entrez Gene 20818 MouseEntrez Gene 300965 RatEntrez Gene 58477 Human
  UniProt ID Q9Y5M8
• Background: The protein encoded by this gene has similarity to mouse protein which is a subunit of the signal recognition particle receptor (SR). This subunit is a transmembrane GTPase belonging to the GTPase superfamily. It anchors alpha subunit, a peripheral membrane GTPase, to the ER membrane. SR is required for the cotranslational targeting of both secretory and membrane proteins to the ER membrane. [provided by RefSeq, Jul 2008]
• Synonyms: APMCF1

Reference Data

• Protein Families: Druggable Genome, Transmembrane