Myeloid leukemia factor 1 (MLF1) Mouse Monoclonal Antibody [Clone ID: OTI5D9]

CAT#: CF504745

Carrier-free (BSA/glycerol-free) MLF1 mouse monoclonal antibody, clone OTI5D9 (formerly 5D9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLF1 (RC202774, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLF1. Positive lysates LY411706 (100ug) and LC411706 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504745)

• 

  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504745)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504745)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504745)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504745)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504745)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504745)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504745)

• HEK293T cells transfected with either RC202774 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MLF1 antibody (TA504745), and then analyzed by flow cytometry.

Show all 10 images

Specifications

Product Data

• Clone Name: OTI5D9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant prtein of human MLF1(NP_071888) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.4 kDa
• Gene Name: Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 1, mRNA.
• Database Link:
  NP_071888
  Entrez Gene 4291 Human
  UniProt ID P58340
• Background: This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: myeloid leukemia factor 1; myeloid leukemia factor 1 variant 1; myeloid leukemia factor 1 variant 2; myeloid leukemia factor 1 variant 3

Reference Data

• Protein Families: Druggable Genome