DBT Mouse Monoclonal Antibody [Clone ID: OTI1G2]

CAT#: CF504748

Carrier-free (BSA/glycerol-free) DBT mouse monoclonal antibody, clone OTI1G2 (formerly 1G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (RC201998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT. Positive lysates LY419653 (100ug) and LC419653 (20ug) can be purchased separately from OriGene.

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  Anti-DBT mouse monoclonal antibody (TA504748) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT (RC201998).

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  HEK293T cells transfected with either RC201998 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DBT antibody (TA504748), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1G2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DBT(NP_001909) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.4 kDa
• Gene Name: Homo sapiens dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.
• Database Link:
  NP_001909
  Entrez Gene 13171 MouseEntrez Gene 29611 RatEntrez Gene 1629 Human
  UniProt ID P11182
• Background: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]
• Synonyms: BCATE2; BCKAD-E2; BCKADE2; BCOADC-E2; E2; E2B

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation