NQO2 Mouse Monoclonal Antibody [Clone ID: OTI1G2]

CAT#: CF504784

Carrier-free (BSA/glycerol-free) NQO2 mouse monoclonal antibody, clone OTI1G2 (formerly 1G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NQO2 (RC202889, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NQO2. Positive lysates LY424463 (100ug) and LC424463 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-NQO2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NQO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504784)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NQO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504784)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NQO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504784)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NQO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504784)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NQO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504784)

• Anti-NQO2 mouse monoclonal antibody (TA504784) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NQO2 (RC202889).

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Specifications

Product Data

• Clone Name: OTI1G2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NQO2(NP_000895) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.7 kDa
• Gene Name: Homo sapiens N-ribosyldihydronicotinamide:quinone reductase 2 (NQO2), transcript variant 3, mRNA.
• Database Link:
  NP_000895
  Entrez Gene 606932 DogEntrez Gene 707675 MonkeyEntrez Gene 4835 Human
  UniProt ID P16083
• Background: NQO2 (EC 1.10.99.2) is a flavoprotein that catalyzes the 2-electron reduction of various quinones, redox dyes, and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al., 1997 [PubMed 9367528]). [supplied by OMIM]
• Synonyms: DHQV; DIA6; NMOR2; QR2