CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: OTI1F9]

CAT#: CF504798

Carrier-free (BSA/glycerol-free) PECAM1 mouse monoclonal antibody,clone OTI1F9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1. Positive lysates LY424716 (100ug) and LC424716 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504798)

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Specifications

Product Data

• Clone Name: OTI1F9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full lenth human recombinant protein of human PECAM1(NP_000433) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.4 kDa
• Gene Name: Homo sapiens platelet and endothelial cell adhesion molecule 1 (PECAM1)
• Database Link:
  NP_000433
  Entrez Gene 5175 Human
  UniProt ID P16284
• Background: The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May
• Synonyms: CD31; EndoCAM; endoCAM; GPIIA; PECA1; PECAM-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration