PADI4 Mouse Monoclonal Antibody [Clone ID: OTI5E7]

CAT#: CF504814

Carrier-free (BSA/glycerol-free) PADI4 mouse monoclonal antibody, clone OTI5E7 (formerly 5E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4. Positive lysates LY402202 (100ug) and LC402202 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504814)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504814)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504814)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA504814) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA504814) (1:500)

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Specifications

Product Data

• Clone Name: OTI5E7
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 299-588 of human PADI4(NP_036519) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.9 kDa
• Gene Name: Homo sapiens peptidyl arginine deiminase 4 (PADI4), mRNA.
• Database Link:
  NP_036519
  Entrez Gene 23569 Human
  UniProt ID Q9UM07
• Background: This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]
• Synonyms: PAD; PAD4; PADI5; PDI4; PDI5