GIMAP4 Mouse Monoclonal Antibody [Clone ID: OTI1C6]

CAT#: CF504849

Carrier-free (BSA/glycerol-free) GIMAP4 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GIMAP4 (RC204653, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GIMAP4. Positive lysates LY413123 (100ug) and LC413123 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GIMAP4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GIMAP4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GIMAP4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GIMAP4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GIMAP4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-GIMAP4 mouse monoclonal antibody (TA504849) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GIMAP4 (RC204653).

• HEK293T cells transfected with either RC204653 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GIMAP4 antibody (TA504849), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1C6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GIMAP4(NP_060796) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.4 kDa
• Gene Name: GTPase, IMAP family member 4
• Database Link:
  NP_060796
  Entrez Gene 55303 Human
  UniProt ID Q9NUV9
• Background: This gene encodes a protein belonging to the GTP-binding superfamily and to the immuno-associated nucleotide (IAN) subfamily of nucleotide-binding proteins. The encoded protein of this gene may be negatively regulated by T-cell acute lymphocytic leukemia 1 (TAL1). In humans, the IAN subfamily genes are located in a cluster at 7q36.1. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: IAN-1; IAN1; IMAP4; MSTP062