DPH2 Mouse Monoclonal Antibody [Clone ID: OTI4E7]

CAT#: CF504852

Carrier-free (BSA/glycerol-free) DPH2 mouse monoclonal antibody, clone OTI4E7 (formerly 4E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPH2 (RC201382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPH2. Positive lysates LY419990 (100ug) and LC419990 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPH2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DPH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504852)

• HEK293T cells transfected with either RC201382 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DPH2 antibody (TA504852), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4E7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DPH2(NP_001375) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.9 kDa
• Gene Name: Homo sapiens diphthamide biosynthesis 2 (DPH2), transcript variant 1, mRNA.
• Database Link:
  NP_001375
  Entrez Gene 67728 MouseEntrez Gene 298452 RatEntrez Gene 475387 DogEntrez Gene 702123 MonkeyEntrez Gene 1802 Human
  UniProt ID Q9BQC3
• Background: This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: DPH2L2