STING (TMEM173) Mouse Monoclonal Antibody [Clone ID: OTI4E12]

CAT#: CF505032

Carrier-free (BSA/glycerol-free) TMEM173 mouse monoclonal antibody, clone OTI4E12 (formerly 4E12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM173 (RC208418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM173. Positive lysates LY405006 (100ug) and LC405006 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505032)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505032)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505032)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505032)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505032)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505032)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TMEM173 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505032)

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Specifications

Product Data

• Clone Name: OTI4E12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TMEM173(NP_938023) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42 kDa
• Gene Name: Homo sapiens transmembrane protein 173 (TMEM173), transcript variant 1, mRNA.
• Database Link:
  NP_938023
  Entrez Gene 340061 Human
  UniProt ID Q86WV6
• Synonyms: ERIS; hMITA; hSTING; MITA; MPYS; NET23; SAVI; STING

Reference Data

• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway