RTKN Mouse Monoclonal Antibody [Clone ID: OTI1A4]

CAT#: CF505051

Carrier-free (BSA/glycerol-free) RTKN mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTKN (RC204517, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTKN. Positive lysates LY403228 (100ug) and LC403228 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RTKN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505051)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RTKN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505051)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RTKN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505051)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RTKN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505051)

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  Anti-RTKN mouse monoclonal antibody (TA505051) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RTKN (RC204517).

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Specifications

Product Data

• Clone Name: OTI1A4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RTKN(NP_149035) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61 kDa
• Gene Name: Homo sapiens rhotekin (RTKN), transcript variant 2, mRNA.
• Database Link:
  NP_149035
  Entrez Gene 20166 MouseEntrez Gene 297383 RatEntrez Gene 6242 Human
  UniProt ID Q9BST9
• Background: This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
• Synonyms: B5