LDOC1 Mouse Monoclonal Antibody [Clone ID: OTI1A2]

CAT#: CF505057

Carrier-free (BSA/glycerol-free) LDOC1 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDOC1 (RC200543, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDOC1. Positive lysates LY402194 (100ug) and LC402194 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1A2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LDOC1(NP_036449) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.8 kDa
• Gene Name: Homo sapiens LDOC1 regulator of NFKB signaling (LDOC1), mRNA.
• Database Link:
  NP_036449
  Entrez Gene 23641 Human
  UniProt ID O95751
• Background: The protein encoded by this gene contains a leucine zipper-like motif and a proline-rich region that shares marked similarity with an SH3-binding domain. The protein localizes to the nucleus and is down-regulated in some cancer cell lines. It is thought to regulate the transcriptional response mediated by the nuclear factor kappa B (NF-kappaB). The gene has been proposed as a tumor suppressor gene whose protein product may have an important role in the development and/or progression of some cancers. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: BCUR1; Mar7; Mart7