Estrogen Related Receptor gamma (ESRRG) Mouse Monoclonal Antibody [Clone ID: OTI1E5]

CAT#: CF505080

Carrier-free (BSA/glycerol-free) ESRRG mouse monoclonal antibody, clone OTI1E5 (formerly 1E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ESRRG (RC218233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ESRRG. Positive lysates LY400557 (100ug) and LC400557 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ESRRG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505080)

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Specifications

Product Data

• Clone Name: OTI1E5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 24-263 of human ESRRG(NP_001429) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.1 kDa
• Gene Name: Homo sapiens estrogen related receptor gamma (ESRRG), transcript variant 1, mRNA.
• Database Link:
  NP_001429
  Entrez Gene 26381 MouseEntrez Gene 360896 RatEntrez Gene 2104 Human
  UniProt ID P62508
• Synonyms: ERR3; ERRgamma; NR3B3

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors