CLEC4E Mouse Monoclonal Antibody [Clone ID: OTI2H4]

CAT#: CF505097

Carrier-free (BSA/glycerol-free) CLEC4E mouse monoclonal antibody, clone OTI2H4 (formerly 2H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLEC4E (RC200244, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLEC4E. Positive lysates LY415339 (100ug) and LC415339 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CLEC4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505097)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CLEC4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505097)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CLEC4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505097)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CLEC4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505097)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLEC4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505097)

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Specifications

Product Data

• Clone Name: OTI2H4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CLEC4E(NP_055173) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.9 kDa
• Gene Name: Homo sapiens C-type lectin domain family 4 member E (CLEC4E), mRNA.
• Database Link:
  NP_055173
  Entrez Gene 26253 Human
  UniProt ID Q9ULY5
• Background: This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: CLECSF9; MINCLE

Reference Data

• Protein Families: Druggable Genome, Transmembrane