LEPRE1 (P3H1) Mouse Monoclonal Antibody [Clone ID: OTI1B4]

CAT#: CF505105

Carrier-free (BSA/glycerol-free) LEPRE1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEPRE1 (RC222535, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEPRE1. Positive lysates LY411682 (100ug) and LC411682 (20ug) can be purchased separately from OriGene.

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  Anti-LEPRE1 mouse monoclonal antibody (TA505105) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LEPRE1 (RC222535).

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  HEK293T cells transfected with either RC222535 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LEPRE1 antibody (TA505105), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1B4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LEPRE1(NP_071751) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83.2 kDa
• Gene Name: Homo sapiens prolyl 3-hydroxylase 1 (P3H1), transcript variant 1, mRNA.
• Database Link:
  NP_071751
  Entrez Gene 64175 Human
  UniProt ID Q32P28
• Background: This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Two alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq]
• Synonyms: GROS1; LEPRE1; OI8

Reference Data

• Protein Families: Secreted Protein