FATE1 Mouse Monoclonal Antibody [Clone ID: OTI5E2]

CAT#: CF505183

Carrier-free (BSA/glycerol-free) FATE1 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FATE1 (RC205951, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FATE1. Positive lysates LY409735 (100ug) and LC409735 (20ug) can be purchased separately from OriGene.

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  Anti-FATE1 mouse monoclonal antibody (TA505183) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FATE1 (RC205951).

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  HEK293T cells transfected with either RC205951 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FATE1 antibody (TA505183), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5E2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FATE1(NP_149076) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.5 kDa
• Gene Name: Homo sapiens fetal and adult testis expressed 1 (FATE1), mRNA.
• Database Link:
  NP_149076
  Entrez Gene 89885 Human
  UniProt ID Q969F0
• Background: This gene encodes a cancer-testis antigen that is highly expressed in hepatocellular carcinomas and other tumors and weakly expressed in normal tissues except testis. The protein is strongly expressed in spermatogonia, primary spermatocytes, and Sertoli cells in seminiferous tubules. This protein may have a role in the control of early testicular differentiation and cell proliferation. [provided by RefSeq]. COMPLETENESS: full length.
• Synonyms: CT43; FATE

Reference Data

• Protein Families: Transmembrane