FATE1 Mouse Monoclonal Antibody [Clone ID: OTI6F10]

CAT#: CF505215

Carrier-free (BSA/glycerol-free) FATE1 mouse monoclonal antibody, clone OTI6F10 (formerly 6F10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FATE1 (RC205951, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FATE1. Positive lysates LY409735 (100ug) and LC409735 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FATE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Anti-FATE1 mouse monoclonal antibody (TA505215) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FATE1 (RC205951).

Specifications

Product Data

• Clone Name: OTI6F10
• Applications: IF, WB
• Recommended Dilution: WB 1:200~4000, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FATE1(NP_001756) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.5 kDa
• Gene Name: Homo sapiens fetal and adult testis expressed 1 (FATE1), mRNA.
• Database Link:
  NP_149076
  Entrez Gene 77905 MouseEntrez Gene 481076 DogEntrez Gene 704368 MonkeyEntrez Gene 89885 Human
  UniProt ID Q969F0
• Synonyms: CT43; FATE

Reference Data

• Protein Families: Transmembrane