ZNF447 (ZSCAN18) Mouse Monoclonal Antibody [Clone ID: OTI2D3]

CAT#: CF505242

Carrier-free (BSA/glycerol-free) ZSCAN18 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN18 (RC203390, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN18. Positive lysates LY402961 (100ug) and LC402961 (20ug) can be purchased separately from OriGene.

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  Anti-ZSCAN18 mouse monoclonal antibody (TA505242) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ZSCAN18 (RC203390).

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  HEK293T cells transfected with either RC203390 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ZSCAN18 antibody (TA505242), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2D3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZSCAN18(NP_076415) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.6 kDa
• Gene Name: Homo sapiens zinc finger and SCAN domain containing 18 (ZSCAN18), transcript variant 3, mRNA.
• Database Link:
  NP_076415
  Entrez Gene 65982 Human
  UniProt ID Q8TBC5
• Synonyms: ZNF447

Reference Data

• Protein Families: Transcription Factors