Serum Amyloid P (APCS) Mouse Monoclonal Antibody [Clone ID: OTI1H2]

CAT#: CF505248

Carrier-free (BSA/glycerol-free) APCS mouse monoclonal antibody, clone OTI1H2 (formerly 1H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APCS (RC202802, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APCS. Positive lysates LY400617 (100ug) and LC400617 (20ug) can be purchased separately from OriGene.

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  Anti-APCS mouse monoclonal antibody (TA505248) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APCS (RC202802).

Specifications

Product Data

• Clone Name: OTI1H2
• Applications: IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human APCS(NP_001630) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.2 kDa
• Gene Name: Homo sapiens amyloid P component, serum (APCS), mRNA.
• Database Link:
  NP_001630
  Entrez Gene 325 Human
  UniProt ID P02743
• Background: The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]
• Synonyms: HEL-S-92n; PTX2; SAP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein