RAB30 Mouse Monoclonal Antibody [Clone ID: OTI3C6]

CAT#: CF505318

Carrier-free (BSA/glycerol-free) RAB30 mouse monoclonal antibody, clone OTI3C6 (formerly 3C6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB30 (RC204318, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB30. Positive lysates LY415246 (100ug) and LC415246 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-RAB30 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RAB30 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505318)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RAB30 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505318)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RAB30 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505318)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RAB30 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505318)

• HEK293T cells transfected with either RC204318 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAB30 antibody (TA505318), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3C6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RAB30(NP_055303) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.9 kDa
• Gene Name: Homo sapiens RAB30, member RAS oncogene family (RAB30), transcript variant 2, mRNA.
• Database Link:
  NP_055303
  Entrez Gene 75985 MouseEntrez Gene 308821 RatEntrez Gene 27314 Human
  UniProt ID Q15771
• Synonyms: member RAS oncogene family; RAB30

Reference Data

• Protein Families: Druggable Genome