EGR2 Mouse Monoclonal Antibody [Clone ID: OTI1F10]

CAT#: CF505319

Carrier-free (BSA/glycerol-free) EGR2 mouse monoclonal antibody,clone OTI1F10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGR2 (RC212183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGR2. Positive lysates LY424742 (100ug) and LC424742 (20ug) can be purchased separately from OriGene.

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  Anti-EGR2 mouse monoclonal antibody (TA505319) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EGR2 (RC212183).

Specifications

Product Data

• Clone Name: OTI1F10
• Applications: IF, WB
• Recommended Dilution: WB 1:500, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 156-476 of human EGR2(NP_000390) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: Homo sapiens early growth response 2 (EGR2), transcript variant 1, mRNA.
• Database Link:
  NP_000390
  Entrez Gene 13654 MouseEntrez Gene 114090 RatEntrez Gene 1959 Human
  UniProt ID P11161
• Background: The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
• Synonyms: AT591; CMT1D; CMT4E; KROX20

Reference Data

• Protein Families: Druggable Genome, Transcription Factors