TSC22D3 Mouse Monoclonal Antibody [Clone ID: OTI1H3]

CAT#: CF505355

Carrier-free (BSA/glycerol-free) TSC22D3 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D3 (RC210088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D3. Positive lysates LY418232 (100ug) and LC418232 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1H3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TSC22D3(NP_004080) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.6 kDa
• Gene Name: Homo sapiens TSC22 domain family member 3 (TSC22D3), transcript variant 2, mRNA.
• Database Link:
  NP_004080
  Entrez Gene 14605 MouseEntrez Gene 1831 Human
  UniProt ID Q99576
• Background: The protein encoded by this gene shares significant sequence identity with the murine TSC-22 and Drosophila shs, both of which are leucine zipper proteins, that function as transcriptional regulators. The expression of this gene is stimulated by glucocorticoids and interleukin 10, and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid and chemokine. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: DIP; DSIPI; GILZ; TSC-22R

Reference Data

• Protein Families: Transcription Factors