ETS2 Mouse Monoclonal Antibody [Clone ID: OTI1H4]

CAT#: CF505372

Carrier-free (BSA/glycerol-free) ETS2 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETS2 (RC207377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETS2. Positive lysates LY401607 (100ug) and LC401607 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-ETS2 monoclonal antibody (1:200).

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ETS2 monoclonal antibody (1:200).

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  Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-ETS2 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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  Figure from citation: Western Blot of ETS2 protein level by using anti-ETS2 antibody in MCECs. Dilution: 1:2000 View Citation

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505372)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505372)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505372)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ETS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505372)

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Specifications

Product Data

• Clone Name: OTI1H4
• Applications: IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 90-349 of human ETS2(NP_005230) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.8 kDa
• Gene Name: Homo sapiens ETS proto-oncogene 2, transcription factor (ETS2), transcript variant 1, mRNA.
• Database Link:
  NP_005230
  Entrez Gene 23872 MouseEntrez Gene 304063 RatEntrez Gene 2114 Human
  UniProt ID P15036
• Background: This gene encodes a transcription factor which regulates genes involved in development and apoptosis. The encoded protein is also a protooncogene and shown to be involved in regulation of telomerase. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
• Synonyms: ETS2IT1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Dorso-ventral axis formation