PPIL3 Mouse Monoclonal Antibody [Clone ID: OTI1D4]

CAT#: CF505381

Carrier-free (BSA/glycerol-free) PPIL3 mouse monoclonal antibody, clone OTI1D4 (formerly 1D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPIL3 (RC217389, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPIL3. Positive lysates LY408889 (100ug) and LC408889 (20ug) can be purchased separately from OriGene.

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  Anti-PPIL3 mouse monoclonal antibody (TA505381) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPIL3 (RC217389).

Specifications

Product Data

• Clone Name: OTI1D4
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPIL3(NP_572028) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18 kDa
• Gene Name: Homo sapiens peptidylprolyl isomerase (cyclophilin)-like 3 (PPIL3), transcript variant PPIL3c
• Database Link:
  NP_572028
  Entrez Gene 70225 MouseEntrez Gene 301432 RatEntrez Gene 53938 Human
  UniProt ID Q9H2H8
• Background: This gene encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: cyclophilin-like protein 3; cyclophilin J; CYPJ; peptidylprolyl cis-trans isomerase-like protein 3; peptidylprolyl isomerase (cyclophilin)-like 3; peptidylprolyl isomerase-like 3; PPIase-like protein 3