CMPK1 Mouse Monoclonal Antibody [Clone ID: OTI1H8]

CAT#: CF505390

Carrier-free (BSA/glycerol-free) CMPK1 mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CMPK1 (RC204856, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CMPK1. Positive lysates LY402539 (100ug) and LC402539 (20ug) can be purchased separately from OriGene.

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  Anti-CMPK1 mouse monoclonal antibody (TA505390) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CMPK1 (RC204856).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CMPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505390)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CMPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505390)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CMPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505390)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CMPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505390)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CMPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505390)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CMPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505390)

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CMPK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-CMPK1 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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Specifications

Product Data

• Clone Name: OTI1H8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CMPK1(NP_057392) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.7 kDa
• Gene Name: Homo sapiens cytidine/uridine monophosphate kinase 1 (CMPK1), transcript variant 1, mRNA.
• Database Link:
  NP_057392
  Entrez Gene 66588 MouseEntrez Gene 298410 RatEntrez Gene 51727 Human
  UniProt ID P30085
• Background: This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Feb 2012]
• Synonyms: CK; CMK; CMPK; UMK; UMP-CMPK; UMPK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism