PCDH7 Mouse Monoclonal Antibody [Clone ID: OTI2C2]

CAT#: CF505422

Carrier-free (BSA/glycerol-free) PCDH7 mouse monoclonal antibody,clone OTI2C2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCDH7 (RC223033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCDH7. Positive lysates LY410093 (100ug) and LC410093 (20ug) can be purchased separately from OriGene.

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  Anti-PCDH7 mouse monoclonal antibody (TA505422) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PCDH7 (RC223033).

Specifications

Product Data

• Clone Name: OTI2C2
• Applications: IF, WB
• Recommended Dilution: WB 1:500, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PCDH7(NP_115833) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 132.4 kDa
• Gene Name: Homo sapiens protocadherin 7 (PCDH7), transcript variant c, mRNA.
• Database Link:
  NP_115833
  Entrez Gene 54216 MouseEntrez Gene 5099 Human
  UniProt ID O60245
• Background: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a protein with an extracellular domain containing 7 cadherin repeats. The gene product is an integral membrane protein that is thought to function in cell-cell recognition and adhesion. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq, Jul 2008]
• Synonyms: BH-Pcdh; BHPCDH; PPP1R120

Reference Data

• Protein Families: Druggable Genome, Transmembrane