GAPDHS Mouse Monoclonal Antibody [Clone ID: OTI1F6]

CAT#: CF505454

Carrier-free (BSA/glycerol-free) GAPDHS mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAPDHS (RC205362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAPDHS. Positive lysates LY402320 (100ug) and LC402320 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505454)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505454)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505454)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505454)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505454)

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Specifications

Product Data

• Clone Name: OTI1F6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinanti protein of human GAPDHS(NP_055179) produced in HEK293T
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.3 kDa
• Gene Name: Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), mRNA.
• Database Link:
  NP_055179
  Entrez Gene 26330 Human
  UniProt ID O14556
• Background: This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq, Jul 2008]
• Synonyms: GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35

Reference Data

• Protein Families: Druggable Genome