POGK Mouse Monoclonal Antibody [Clone ID: OTI2D5]

CAT#: CF505548

Carrier-free (BSA/glycerol-free) POGK mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POGK (RC222119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POGK. Positive lysates LY413730 (100ug) and LC413730 (20ug) can be purchased separately from OriGene.

• 

  Anti-POGK mouse monoclonal antibody (TA505548) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POGK (RC222119).

• 

  HEK293T cells transfected with either RC222119 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POGK antibody (TA505548), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2D5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human POGK(NP_060012) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69.3 kDa
• Gene Name: Homo sapiens pogo transposable element derived with KRAB domain (POGK), transcript variant 1, mRNA.
• Database Link:
  NP_060012
  Entrez Gene 57645 Human
  UniProt ID Q9P215
• Background: The exact function of the protein encoded by this gene is not known. However, this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus, and a transposase domain at the C-terminus, suggesting that it may belong to the family of DNA-mediated transposons in human. [provided by RefSeq]
• Synonyms: BASS2; KRBOX2; LST003

Reference Data

• Protein Families: Transcription Factors