CD272 (BTLA) Mouse Monoclonal Antibody [Clone ID: OTI2G2]

CAT#: CF505558

Carrier-free (BSA/glycerol-free) BTLA mouse monoclonal antibody, clone OTI2G2 (formerly 2G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA (RC219458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTLA. Positive lysates LY403628 (100ug) and LC403628 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC219458 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTLA antibody (TA505558), and then analyzed by flow cytometry.

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  Anti-BTLA mouse monoclonal antibody (TA505558) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTLA (RC219458).

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  Western blot analysis of extracts (35ug) from PBMCs by using anti-BTLA monoclonal antibody (1:500).

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  Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-BTLA monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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Specifications

Product Data

• Clone Name: OTI2G2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.6 kDa
• Gene Name: Homo sapiens B and T lymphocyte associated (BTLA), transcript variant 1, mRNA.
• Database Link:
  NP_861445
  Entrez Gene 151888 Human
  UniProt ID Q7Z6A9
• Background: BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4. [supplied by OMIM]
• Synonyms: BTLA1; CD272

Reference Data

• Protein Families: Transmembrane