CA12 Mouse Monoclonal Antibody [Clone ID: OTI4G3]

CAT#: CF505610

Carrier-free (BSA/glycerol-free) CA12 mouse monoclonal antibody, clone OTI4G3 (formerly 4G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA12 (RC204810, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CA12. Positive lysates LY400487 (100ug) and LC400487 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CA12 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:2000).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CA12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505610)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CA12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505610)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CA12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505610)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CA12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505610)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CA12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505610)

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Specifications

Product Data

• Clone Name: OTI4G3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CA12(NP_001209) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.8 kDa
• Gene Name: Homo sapiens carbonic anhydrase 12 (CA12), transcript variant 1, mRNA.
• Database Link:
  NP_001209
  Entrez Gene 771 Human
  UniProt ID O43570
• Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CAXII; HsT18816

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Nitrogen metabolism