ROMO1 Mouse Monoclonal Antibody [Clone ID: OTI1G4]

CAT#: CF505612

Carrier-free (BSA/glycerol-free) ROMO1 mouse monoclonal antibody,clone OTI1G4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROMO1 (RC210655, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROMO1. Positive lysates LY409065 (100ug) and LC409065 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ROMO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505612)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ROMO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505612)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ROMO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505612)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ROMO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505612)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ROMO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505612)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ROMO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505612)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ROMO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505612)

• Anti-ROMO1 mouse monoclonal antibody (TA505612) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ROMO1 (RC210655).

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Specifications

Product Data

• Clone Name: OTI1G4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ROMO1(NP_542786) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens reactive oxygen species modulator 1 (ROMO1), mRNA.
• Database Link:
  NP_542786
  Entrez Gene 140823 Human
  UniProt ID P60602
• Synonyms: bA353C18.2; C20orf52; MTGM; MTGMP

Reference Data

• Protein Families: Transmembrane