PARVB Mouse Monoclonal Antibody [Clone ID: OTI1B9]

CAT#: CF505679

Carrier-free (BSA/glycerol-free) PARVB mouse monoclonal antibody, clone OTI1B9 (formerly 1B9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARVB (RC208490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARVB. Positive lysates LY415629 (100ug) and LC415629 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PARVB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:400

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PARVB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505679)

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  Anti-PARVB mouse monoclonal antibody (TA505679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PARVB (RC208490).

Specifications

Product Data

• Clone Name: OTI1B9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PARVB(NP_037459) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.5 kDa
• Gene Name: Homo sapiens parvin beta (PARVB), transcript variant 2, mRNA.
• Database Link:
  NP_037459
  Entrez Gene 170736 MouseEntrez Gene 362973 RatEntrez Gene 29780 Human
  UniProt ID Q9HBI1
• Background: This gene encodes a member of the parvin family of actin-binding proteins, which play a role in cytoskeleton organization and cell adhesion. These proteins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. This family member binds to alphaPIX and alpha-actinin, and it can inhibit the activity of integrin-linked kinase. This protein also functions in tumor suppression. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]
• Synonyms: CGI-56

Reference Data

• Protein Pathways: Focal adhesion