CD272 (BTLA) Mouse Monoclonal Antibody [Clone ID: OTI3D11]

CAT#: CF505687

Carrier-free (BSA/glycerol-free) BTLA mouse monoclonal antibody, clone OTI3D11 (formerly 3D11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA (RC219458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTLA. Positive lysates LY403628 (100ug) and LC403628 (20ug) can be purchased separately from OriGene.

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  Anti-BTLA mouse monoclonal antibody (TA505687) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTLA (RC219458).

Specifications

Product Data

• Clone Name: OTI3D11
• Applications: IF, WB
• Recommended Dilution: WB 1:4000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.6 kDa
• Gene Name: Homo sapiens B and T lymphocyte associated (BTLA), transcript variant 1, mRNA.
• Database Link:
  NP_861445
  Entrez Gene 151888 Human
  UniProt ID Q7Z6A9
• Synonyms: BTLA1; CD272

Reference Data

• Protein Families: Transmembrane